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Knockdown of vascular endothelial cell growth factor and sensitivity of U251 cells to radiotherapy and chemotherapy
Pages: 718-721
Year: Issue:  4
Journal: Chinese Journal of Experimental Surgery

Keyword:  GlioblastomaVascular endothelial growth factorRNA interferenceRadiotherapyChemotherapy;
Abstract: Objective To explore the effect of vascular endothelial growth factor (VEGF) mRNA interference on glioblatoma.Methods After knockdown of VEGF mRNA expression using VEGF short hairpin RNA (shRNA),glioma U251 cell were treated with chemotherapy or radiotherapy or chemotherapy plus radiotherapy or without any treatment.The changes in cell cycle,apoptosis rate,cell colony-formation and cell morphology were observed.Results After knockdown of VEGF mRNA expression using VEGF shRNA,VEGF mRNA expression levels in glioma U251 cells were inhibited significantly (P < 0.001).The flow cytometry revealed that both control cells and VEGF shRNA-transfected cells exhibited G0-G1 arrest,and reduced number of cells in the G2 and M phases.The apoptosis rate of VEGF shRNA-transfected cells was increased as compared with the control cells.It was found that various concentrations of paciltaxel reduced U251 cell viability 50% inhibitory dose(IC50) =28.1 mg/L,and VEGF knockdown significantly sensitized U251 cells to paclitaxel (IC50 =0.02 mg/L).Groups with drug treatment alone and the radiotherapy alone showed no significant difference (P > 0.05).After VEGF knockdown,colony formation in paclitaxel and radiation treated U251 cells was significantly reduced (17.57% to 6.33%,P < 0.01).Under the microscopy,it was found VEGF shRNA-transfected cells showed worse cellular morphology than non-transfected cells.Conclusion The interference of VEGF gene expression can inhibit the proliferation of U251cells,promote apoptosis of U251 cells,and increase the sensitivity of U251 cells to chemotherapy and radiotherapy.Liposomal paclitaxel can enhance drug delivery in vivo,although the VEGF gene interference and the practical application of liposomal paclitaxel remains to be tested and explored in future studies.
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