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Roles of integrin αvβ3 in interleukin-8-mediated metastasis and angiogenesis of estrogen receptor negative breast cancer cells
Pages: 658-660
Year: Issue:  4
Journal: Chinese Journal of Experimental Surgery

Keyword:  Breast neoplasmsEstrogen receptorInterleukin-8Integrin;
Abstract: Objective To identify the role of integin αvβ3 in interleukin (IL)-8-mediated metastasis and angiogenesis of breast cancer cells.Methods IL-8 and neutralizing antibody of integrin αvβ3 were used in invasion and human umbilical vein endothelial cells (HUVECs)-tumor cells adhesion assay to observe the changes of metastasis of breast cancer cells.Proliferation assay and angiogenesis of HUVECs cultured with IL-8 and antibody of αvβ3 were applied to evaluate the effect of αvβ3 on IL-8-mediated angiogenesis.The correlation between IL-8 and αvβ3 expression was examined by using immunohistochemistry,and relationship between the expression of IL-8 and αvβ3 with estrogen receptor (ER) status in patients was further analyzed.Results IL-8 significantly inhanced the migration of ER negative breast cancer cell lines BT-549 and MDA-MB-231 as compared with those cultured with phosphate buffer (PBS,P<0.05).After the addition of antibody of αvβ3,the number of BT-549 and MDA-MB-231 cells cultured with IL-8 was declined significantly (P <0.01),whereas IL-8 had no effect on ER positive breast cancer cell line MCF-7 (P > 0.05).Proliferation of HUVECs cultured with IL-8 was markedly promoted (P <0.01),which could be blocked by antibody of αvβ3 (P <0.01).HUVECs cultured with IL-8 tended to form more microangioid structure,and suppression of angiogenesis could be observed after the addition of antibody of αvβ3.In the 104 breast cancer specimens,62.1% showed positive αvβ3 expression in the IL-8 positive group,which was significantly higher than that in IL-8 negative group (P < 0.01).In addition,the expression of αvβ3 was correlated with IL-8 in ER-negative breast cancer cells (P < 0.05).Conclusion Integrin αvβ3 may be involved in metastasis and angiogenesis of ER-negative breast cancer cells promoted by IL-8.The expression of αvβ3 correlates with IL-8 expression,which is related to ER status.
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