Role of chemokine CCL28 in hypoxia-induced migration of hepatocellular carcinoma
Author(s): ZHOU Ying, ZHANG Bo-heng, YIN Xin, REN Zheng-gang
Pages: 524-527
Year: 2013 Issue: 7
Journal: Chinese Journal of Hepatology
Keyword: Carcinoma; hepatocellular; Chemokine; CCL28; Hypoxia inducible factor-1 α; Migration;
Abstract: Objective To investigate the role of CCL28 in hypoxia-induced cell migration of hepatocellular carcinoma (HCC).Methods Resected liver tissues from 50 HCC patients were subjected to real-time (rt)-PCR analysis to evaluate the mRNA expression levels of the hypoxia-induced factor HIF-1α and the chemokine CCL28.Patient data on treatment and outcome were analyzed.The human HCC cell lines HepG2 and HCCLM3 were used to investigate effects of hypoxic conditions on HIF-1α and CCL28 expressions by rtPCR,western blotting,and enzyme-linked immunoassay.The CCL28-mediated effects of hypoxic conditions on cell mobility and invasion were assessed by trans-well and matrigel assays,respectively,in HCCLM3 with CCL28 expression silenced by small-interferring (si)RNA transfection.Spearman's rank test was used to assess the correlation between CCL28 and effects on disease-and treatment-related factors.Results The mRNA levels of CCL28 (0.025 ± 0.075) were found to be strongly correlated with HIF-1α(0.065 ± 0.098) in human clinical samples of HCC (r =0.595,P ＜ 0.01),with higher expressions of both related to recurrence after surgery (P=0.011 and 0.019,respectively).In vitro hypoxic conditions stimulated HIF-1α and CCL28 expression in a time-dependent manner in both HepG2 (HIF-1α:F=873.5; CCL28:F=151.6) and HCCLM3 (HIF-1α:F =964.5; CCL28:F =285.8) (allP ＜ 0.01).siRNA inhibition of CCL28 in HCCLM3 cells led to a significant reduction in hypoxia-induced invasion and migration (all P =0.011).Conclusion Chemokine CCL28 expression is up-regulated in human HCC and under in vitro hypoxic conditions,and may play an important role in hypoxia-induced HCC migration and invasion.
Pages: 524-527
Year: 2013 Issue: 7
Journal: Chinese Journal of Hepatology
Keyword: Carcinoma; hepatocellular; Chemokine; CCL28; Hypoxia inducible factor-1 α; Migration;
Abstract: Objective To investigate the role of CCL28 in hypoxia-induced cell migration of hepatocellular carcinoma (HCC).Methods Resected liver tissues from 50 HCC patients were subjected to real-time (rt)-PCR analysis to evaluate the mRNA expression levels of the hypoxia-induced factor HIF-1α and the chemokine CCL28.Patient data on treatment and outcome were analyzed.The human HCC cell lines HepG2 and HCCLM3 were used to investigate effects of hypoxic conditions on HIF-1α and CCL28 expressions by rtPCR,western blotting,and enzyme-linked immunoassay.The CCL28-mediated effects of hypoxic conditions on cell mobility and invasion were assessed by trans-well and matrigel assays,respectively,in HCCLM3 with CCL28 expression silenced by small-interferring (si)RNA transfection.Spearman's rank test was used to assess the correlation between CCL28 and effects on disease-and treatment-related factors.Results The mRNA levels of CCL28 (0.025 ± 0.075) were found to be strongly correlated with HIF-1α(0.065 ± 0.098) in human clinical samples of HCC (r =0.595,P ＜ 0.01),with higher expressions of both related to recurrence after surgery (P=0.011 and 0.019,respectively).In vitro hypoxic conditions stimulated HIF-1α and CCL28 expression in a time-dependent manner in both HepG2 (HIF-1α:F=873.5; CCL28:F=151.6) and HCCLM3 (HIF-1α:F =964.5; CCL28:F =285.8) (allP ＜ 0.01).siRNA inhibition of CCL28 in HCCLM3 cells led to a significant reduction in hypoxia-induced invasion and migration (all P =0.011).Conclusion Chemokine CCL28 expression is up-regulated in human HCC and under in vitro hypoxic conditions,and may play an important role in hypoxia-induced HCC migration and invasion.
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