Effect of N-trimethyl Chitosan-coated PLGA Nanoparticles on Antigen Cross-presentation
Author(s): Han Ruiling, Guo Xianxi, Yue Yuan, Ding Hong
Pages: 794-797
Year: 2013 Issue: 6
Journal: China Pharmacist
Keyword: N-trimethy chitosan; PLGA; Nanoparticles; Cross-presentation;
Abstract: 目的:探讨N-三甲基壳聚糖包衣聚乳酸-羟基乙酸共聚物[poly(lactic-co-glycolic acid),PLGA]纳米粒对诱导树突细胞交叉递呈的机制.方法:复乳法制备PLGA纳米粒并用N-三甲基壳聚糖(N-trimethyl chitosan,TMC60)进行包衣,分别包载模型抗原卵清白蛋白、异硫氰酸荧光素(fluorescein isothiocyanate,FITC)标记的卵清白蛋白(FITC-OVA).将TMC60包衣和未包衣的纳米粒分别作用于体外培养的小鼠骨髓系树突细胞(murine bone marrow-derived dendritic cell,BMDC),用流式细胞仪检测BMDC对纳米粒子的吞噬动力学及BMDC表面分子CD83,MHCⅠ和MHCⅡ的表达;B3Z T细胞检测纳米粒被BMDC摄取后引起的交叉递呈反应.结果:TMC60包衣PLGA纳米粒可以促进BMDC吞噬作用;促进BMDC表达表面分子CD83和MHC Ⅰ;增强BMDC对纳米粒包载抗原的交叉递呈作用.结论:TMC60包衣PLGA纳米粒可增强BMDC对外源性抗原的摄取及交叉递呈作用.
Pages: 794-797
Year: 2013 Issue: 6
Journal: China Pharmacist
Keyword: N-trimethy chitosan; PLGA; Nanoparticles; Cross-presentation;
Abstract: 目的:探讨N-三甲基壳聚糖包衣聚乳酸-羟基乙酸共聚物[poly(lactic-co-glycolic acid),PLGA]纳米粒对诱导树突细胞交叉递呈的机制.方法:复乳法制备PLGA纳米粒并用N-三甲基壳聚糖(N-trimethyl chitosan,TMC60)进行包衣,分别包载模型抗原卵清白蛋白、异硫氰酸荧光素(fluorescein isothiocyanate,FITC)标记的卵清白蛋白(FITC-OVA).将TMC60包衣和未包衣的纳米粒分别作用于体外培养的小鼠骨髓系树突细胞(murine bone marrow-derived dendritic cell,BMDC),用流式细胞仪检测BMDC对纳米粒子的吞噬动力学及BMDC表面分子CD83,MHCⅠ和MHCⅡ的表达;B3Z T细胞检测纳米粒被BMDC摄取后引起的交叉递呈反应.结果:TMC60包衣PLGA纳米粒可以促进BMDC吞噬作用;促进BMDC表达表面分子CD83和MHC Ⅰ;增强BMDC对纳米粒包载抗原的交叉递呈作用.结论:TMC60包衣PLGA纳米粒可增强BMDC对外源性抗原的摄取及交叉递呈作用.
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