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jie zhong chan kuai su leng que fa
Author(s): 
Pages: 39
Year: Issue:  2
Journal: Edible Fungi of China

Keyword:  接种箱酒精灯火焰灭菌快速冷却点燃燃烧做法烧红内装插入;
Abstract: <正> 做法是:在接种箱内的酒精灯旁,放置一长形小瓶,内装75%酒精。经酒精灯火焰烧红的接种铲插入75%酒精瓶中,稍停数秒接种铲很快冷却。取出接种铲通过火焰,点燃后立即移开,沾附在接种铲上的酒精燃烧后,自行熄灭。此时,接种铲经火焰灭菌,又经75%酒精灭菌,再点燃沾附在接种铲上的酒精三次灭菌,完全达到无菌效果,而且接种铲不热。经此法灭菌的接种铲可连续移接数次,再如法灭菌。此法既保证接种铲灭
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