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ji xing zuo ran du dui he nan hua xi xie jing zi zhi liang de ying xiang
Author(s): 
Pages: 2044-2049
Year: Issue:  7
Journal: Acta Scientiae Circumstantiae

Keyword:  Sinopotamon henanense spermviabilityplasma- and acrosomal-membrane integritychromatin integritycadmium;
Abstract: 采用急性毒性实验方法,研究了镉(Cd2+)对河南华溪蟹(Sinopotamon henanense)精子质量的影响.实验设置了5个Cd2+浓度组(7.25、14.5、29、58和116mg·L-1)和1个空白对照组,在2个染毒时间(5d和7d)采用特异性的荧光染料和流式细胞术(FCM)对精子成活率、膜完整性、顶体完整性及染色质结构进行了测定.结果显示,随着Cd2+浓度的增加和染毒时间的延长,精子的成活率下降,质膜和顶体缺失率上升,异染色质所占比率上升.在Cd2+浓度为58、116mg·L-1条件下暴露5d后,精子成活率显著降低(p<0.05);Cd2+浓度为116mg·L-1时,精子质膜完整性和染色质结构均有明显的损伤;而顶体状态在Cd2+浓度为29mg·L-1时就呈现显著的损伤(p<0.01).在Cd2+浓度为29、58、116mg·L-1条件下染毒7d后,精子成活率、质膜完整性和顶体完整性降低;在所有的染毒组,DNA结构受到了显著的破坏.结果表明,Cd2+暴露对华溪蟹精子质量有明显的影响.
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