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Synthesis and Cloning of the Tandem Repeated Genes for Hammerhead Ribozyme and Antisense RNA Against Melon mRNA for ACC Oxidase
Author(s): 
Pages: 557-561
Year: Issue:  5
Journal: ACTA SCIENTIARUM NATURALIUM UNIVERSITATIS NEIMENGGOL

Keyword:  蜜瓜ACC氧化酶核酶反义RNA;
Abstract: 我们设计和合成了切割蜜瓜成熟果实ACC氧化酶mRNA的GUC86、GUC388、GUC534的三重锤头型核酶(HhRz)和阻断转录的反义RNA(AR)基因,并构建了二、四、八联体HhRz和AR基因.为了在体外和体内检测HhRz是否切割ACC氧化酶mRNA,在E.coli DE3rna-菌株中克隆了同域和跨域作用的(HhRz)n-AR和靶子ACC氧化酶mRNA表达系统.又构建了植物体组成型和诱导型(HhRz)n-AR表达载体.
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