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THE SECOND INTERNAL TRANSCRIBED SPACER OF NUCLEAR RIBOSOMAL DNA AS A MOLECULAR IDENTITY FOR CHINESE ANOPHELINE TAXONOMY:I SUBGENUS ANOPHELES SPECIES
Author(s): 
Pages: 16-24
Year: Issue:  1
Journal: Acta Parasitology et Medica Entomologica Sinica

Keyword:  rDNA-ITS2按蚊亚属鉴别评述;
Abstract: 对核糖体DNA第2内转录间隔区(rDNA-ITS2)序列作为中国按蚊属按蚊亚属蚊种鉴别的分子特征进行评价.笔者整理和分析了GenBank数据库中注册的所有中国记录的按蚊亚属蚊种的rDNA-ITS2序列,并应用MEGA软件计算其在种内和种间的差异性(p距离).截止2012年10月,GenBank中已注册ITS2序列共584条,隶属按蚊亚属蚊20种(包括同物异名)、待定种5种和杂交种1个.计算结果显示,ITS2序列在种内的变异性小于1%,种间的差异(掣 为1.2%(凉山按蚊与昆明按蚊)~69.5%(待定种YM-2003a与须喙按蚊).另外,发现少数注册(、-矽)列在种内差异性较大,在比对序列时需谨慎.结果提示rDNA-ITS2序列在本研究按蚊中具有良好的种内保守性和种间解析度.
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