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Cloning, expression and activity determination of capsid protein VP1 of enterovirus type 71
Author(s): HUANG Xueyong, LI Xingle, HU Xiaoning, DU Yanhua, XU Yuling, WEI Haiyan, CHEN Haomin, XU Zuoli
Pages: 541-
542
Year: 2013
Issue:
5
Journal: Chinese Journal of Epidemiology
Keyword: Enterovirus type 71; Capsid protein VP1; Prokaryotic express; Antigenicity;
Abstract: 肠道病毒71型(EV71)和柯萨奇病毒A组16型(CoxA16)是引起手足口病最为常见肠道病毒,其中EV71是引起当前我国手足口病的病原优势株[1].目前临床对EV71感染以对症治疗为主,尚无有效的抗病毒药物,研制安全有效的疫苗和早期诊断试剂是预防控制手足口病疫情的经济有效地措施,为此进行以下相关研究.
1.材料与方法:
(1)材料:EV71/Henan/106/2009分离株(HQ998852.1)由本实验室从郑州市手足口病患儿粪便标本中分离并保种.手足口病患儿急性期血清和健康儿童血清标本采集于郑州市儿童医院.
Citations
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