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The experimental study of two bivalent Shigella vaccines immunized mice in different mucosal
Author(s): 
Pages: 523-526
Year: Issue:  5
Journal: CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY

Keyword:  痢疾疫苗滴鼻免疫灌胃免疫肠内免疫抗体分泌细胞;
Abstract: 目的探讨不同粘膜免疫途径对2株痢疾工程疫苗免疫效果的影响. 方法用FSM-2117或FS-5416以4×107CFU/只分别经滴鼻、灌胃和小肠内免疫小鼠,3次免疫后7?d分离小鼠脾、派伊尔结(PP)、肠系膜中心淋巴结(MLN)、小肠淋巴细胞固有层(LPL)、鼻通道(NP)及NALT淋巴细胞,用BA-ELISASPOT法检测其特异性抗FSM-2117或FS-5416全菌抗原的IgA、IgG-ASC(抗体分泌细胞)数量. 结果 2株疫苗经鼻内免疫都诱发了鼻粘膜相关淋巴组织、胃肠粘膜相关淋巴组织以及代表系统免疫反应的脾淋巴细胞全菌抗原特异性IgA、IgG-ASC的显著增加(P<0.05和0.01).小肠内免疫后,可有效诱发肠粘膜局部以及脾淋巴细胞全菌抗原特异性IgA、IgG-ASC的显著增加(P<0.01),但不能刺激鼻粘膜局部淋巴细胞的特异性ASC反应. 结论 2株疫苗经鼻粘膜免疫均可诱导NALT和GALT及系统免疫反应的发生,是一个可行的免疫途径.
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