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lian he fa leng dong bao cun ri ben xue xi chong tong chong de yan jiu
Author(s): 
Pages: 35-37
Year: Issue:  6
Journal: Chinese Veterinary Science

Keyword:  日本血吸虫童虫联合法冷冻保存三滴法铝盘速冻法移液器冷冻方法旋涡吊桶;
Abstract: <正> James(1981)首先提出了冷冻保存曼氏血吸虫的乙二醇二步加入速冻法,Stirewalt等(1984)认为该法很好,但培养大量童虫并加以冷冻却有困难,因此作了改良。我们采用该二氏的基本方法,结合本实验室条件,用乙二醇二步加速冻法保存日本血吸虫童虫的研究中,取得了解冻后形态正常并活动的童虫最多占44.68%的试验结果。在此基础上,进一步找出了本文报道的“联合法”。此法可以培养大量童虫,并可在冰冻状态下进行童虫的辐照。
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