bi ma can zai bian tai qi jian dai xie zuo yong de yan jiu . suan xing lin suan yi zuo mei de xing zhi ji qi huo li zai bian tai qi de bian hua
Author(s): FENG HUI Institute of Zoology, Academia Sinica
Pages: 815-823
Year: 1964 Issue: 6
Journal: Acta Entomologica Sinica
Keyword: 血淋巴; 酶活力; 蓖麻蚕; 变态期; 磷酸一酯; 酶的性质; 脂肪体; 代谢作用; 醋酶; 酸性;
Abstract: During metamorphosis the activity of the hemolymph phosphomonoesterase of Eri-silkworm changes in a regular pattcrn according to the developmental stages. In thebeginning of the last larval instar, on the fourth day of the fifth instar and in the pre-pupal stage the enzymic activity is the highest; it decreases sharply just after pupationand increases again before and after emergence. The enzymic activity calculated perunit volume of hemolymph shows no significant difference between the sexes. However,from the middle of the fifth instar, the enzymic activity calculated per unit weight ofprotein of the males is always higher than that of the females. The distribution andchange of activities of this enzyme between different tissues are as follows: In themiddle of the fifth instar larvae, the enzyme activity in the digestive tract is higher thanthose of other parts of the body, that of the mid-gut being the highest. The lowestvalues of enzymic activity was found in the fat bodies and silk glands. The enzymeactivity in the fat bodies increased in the pupal stage, reached the highest level afteremergence. These facts indicate that in the middle of fifth larval instar the mid-gut, where active phosphorylation and dephosphorylation are carried out, is theactive site of metabolism and that in the pupa and adult stages when the digestivetract degenerates, the fat bodies play an important role in metabolism. The hemolymphof insects differs from the blood of other animals by a high content of phosphates. Thetypes and the amounts of phosphates in the hemolymph are found to be influenced bythe selective bydrolysis in the hemolymph and the selective secretion from the cells, inagreement with the view expressed by Wyatt (1963). During metamorphosis the selec-tive hydrolysis is effected by the presence and changes of activities of the phosphataseswith different specificities. The present study further indicates that the mid-gut and thefat bodies of the fifth instar larvae and the fat bodies of the pupae and adult are themain sources of hemolymph phosphates. The properties of hemolymph acid phospho-monoesterase and the inhibition and activation of enzymic activities by some metal ions,organic acids, etc., have also been studied.
Pages: 815-823
Year: 1964 Issue: 6
Journal: Acta Entomologica Sinica
Keyword: 血淋巴; 酶活力; 蓖麻蚕; 变态期; 磷酸一酯; 酶的性质; 脂肪体; 代谢作用; 醋酶; 酸性;
Abstract: During metamorphosis the activity of the hemolymph phosphomonoesterase of Eri-silkworm changes in a regular pattcrn according to the developmental stages. In thebeginning of the last larval instar, on the fourth day of the fifth instar and in the pre-pupal stage the enzymic activity is the highest; it decreases sharply just after pupationand increases again before and after emergence. The enzymic activity calculated perunit volume of hemolymph shows no significant difference between the sexes. However,from the middle of the fifth instar, the enzymic activity calculated per unit weight ofprotein of the males is always higher than that of the females. The distribution andchange of activities of this enzyme between different tissues are as follows: In themiddle of the fifth instar larvae, the enzyme activity in the digestive tract is higher thanthose of other parts of the body, that of the mid-gut being the highest. The lowestvalues of enzymic activity was found in the fat bodies and silk glands. The enzymeactivity in the fat bodies increased in the pupal stage, reached the highest level afteremergence. These facts indicate that in the middle of fifth larval instar the mid-gut, where active phosphorylation and dephosphorylation are carried out, is theactive site of metabolism and that in the pupa and adult stages when the digestivetract degenerates, the fat bodies play an important role in metabolism. The hemolymphof insects differs from the blood of other animals by a high content of phosphates. Thetypes and the amounts of phosphates in the hemolymph are found to be influenced bythe selective bydrolysis in the hemolymph and the selective secretion from the cells, inagreement with the view expressed by Wyatt (1963). During metamorphosis the selec-tive hydrolysis is effected by the presence and changes of activities of the phosphataseswith different specificities. The present study further indicates that the mid-gut and thefat bodies of the fifth instar larvae and the fat bodies of the pupae and adult are themain sources of hemolymph phosphates. The properties of hemolymph acid phospho-monoesterase and the inhibition and activation of enzymic activities by some metal ions,organic acids, etc., have also been studied.
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