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sheng wu cai liao zhong wei liang ti de bi se ce ding fa
Pages: 167-173
Year: Issue:  2
Journal: Acta Physiologica Sinica

Keyword:  生物材料微量锑生物样品比色测定法消化作用混合液酸溶液分光光度硫酸纳毫升;
Abstract: A rapid and accurate micro-colorimetric method for the determination of antimony in biological materials is described.A sample of whole blood,urine or animal tissue containing antimony is digested with the aid of heating in perchloric acid-nitric acid mixture,followed by the addition of concentrated sulfuric acid.Additional amounts of concentrated nitric acid are frequently added until the digestion is complete.After boiling with 1% Na_2SO_3,the ice-cold mixture of the digest is treated with an aqueous solution of NaCl and Ce(SO_4)_2,and then ex- tracted with amyl acetate.The amyl acetate layer is shaken with a solution containing 0.5 mg rhodamine B in 2.5 N HCl to give a rosy color.The color of the centrifuged extract is compared in a spectrophotometer(555 mμ)or a colorimeter(green filter).When a sample contains antimony in excess of 6 6 μg,it is advisable to shake the extract once more with twice its volume of a rhodamine B solution containing 1 mg of the dye in 2.5 N HCl,and to compare the color after the extract is diluted with amyl acetate previously shaken with 2.5 N HCl. The determination is rapid and only about one hour is required to digest 10—20 samples at the same time.As small as 0.2 μg of antimony in a sample of biological material can be deter- mined.Even with such a small quantity of antimony,the error of determination is usually 3—5% and seldom reaches 7%. The above method has been successfully applied to the estimation of antimony in the urine and blood of patients with scistosomiasis in the course of tartar emetic treatment,and also to the determination of antimony in the tissues of rabbits treated with tartar emetic.
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