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15 jia ji qian lie xian su f2 dui tu luan chao huan lin suan xian zuo he yun tong han liang de ying xiang
Author(s): 
Pages: 50-54
Year: Issue:  1
Journal: Acta Physiologica Sinica

Keyword:  孕酮含量前列腺素FcAMP含量静脉注射卵巢细胞环磷酸茶碱子宫卵巢静脉溶黄体作用腺苷;
Abstract: 15-methyl-PGF_(2α),HCG,HCG and 15-methyl-PGF_(2α),theophylline,theophyl-line and 15-methyl-PGF_(2α)were given separately as intravenous injections to pseudo-pregnant rabbits.Sixty minutes after administration of the drugs,the rabbitswere anaesthetized with sodium pentobarbital and the ovaries were taken outand assayed for cAMP and progesterone.In the group after 15-methyl-PGF_(2α)injection,the progesterone and cAMPcontents of the ovary decreased obviously compared with those of the control group.However,when HCG was given intravenously,remarkable increases in both theprogesterone and cAMP contents of the ovary were produced.It is well known thatcAMP is the second messenger for LH action in stimulating the synthesis of pro-gesterone by the ovary.Following the combined administration of HCG with15-methyl-PGF_(2α),the progesterone and cAMP contents of ovary did not increaseand were similar to those of the control group.This shows that the effects of HCGin stimulating synthesis of progesterone and increasing the content of cAMP inthe ovarian tissue might be antagonized by 15-methyl-PGF_(2α).The result sug-gests that the mechanism of luteolytic action of 15-methyl-PGF_(2α)appears to berelated to the lowering of the cAMP level of the ovary.After theophylline was administered,it caused a significant rise of the levelof progesterone and cAMP in the ovarian tissue.Since theophylline is an inhibitorof phosphodiesterase,this result might indicate that in the pseudo-pregnant rabbitovary endogenous cAMP is inactivated by phosphodiesterase,and that the con-version of endogenous cAMP into 5'-AMP by phosphodiesterase may be a regula-tory mechanism in the control of ovarian progesterone synthesis.In the groupgiven theophylline and 15-methyl-PGF_(2α)simultaneously the progesterone and cAMPcontents of ovary were similar to those of control group.This shows that the effectof theophylline in stimulating the synthesis of progesterone and increasing the con-tent of cAMP in the ovary might be antagonized by 15-methyl-PGF_(2α).This resultfurther supports the assumption that the mechanism of luteolysis by 15-methyl-PGF_(2α) is probably closely related to lowering the cAMP level in the ovarian tis-sue.
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