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xiao shu shen jing xi tong nei qiang fei zuo b mian yi huo xing wu zhi ji qi gao ya ye xiang ceng xi (hplc) fen xi
Pages: 116-122
Year: Issue:  2
Journal: Acta Physiologica Sinica

Keyword:  dynorphin Bdynorphin-32dynorphin B-29radioimmunoassayhigh performaceliquid chromatographpituitary;
Abstract: Dynorphin B is a novel opioid tridecapeptide. We have developed a sensitiveradioimmunoassay for dynorphin B and its related peptides (dynorphin-32 anddynorphin B-29) and determined the immunoreactive dynorphin B levels in pitui-tary and some other areas of mouse central nervous system. The highest concentra-tions were found in pituitary and hypothalamus. Sephadex G-50 chromatography and HPLC reveal that immunoreactivedynorphin B in mouse brain mainly is dynorphin B, and also comprises certainamount of dynorphin-32 and an unknown component with higher molecular weight.
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