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lin suan peng suan huan chong ye dian yong ( zhai yao )
Author(s): 
Pages: 8
Year: Issue:  4
Journal: Acta Universitatis Medicinalis Nanjing

Keyword:  缓冲液对流电泳优点醋纤膜电泳硼酸磷酸巴比妥蒸馏水血清工作液;
Abstract: <正> 鉴于常用的巴比妥缓冲液尚有缺点,特别在室温高于33℃时作线电泳其线条往往不清。因此提出用磷酸一硼酸缓冲液作线电泳等,具有安全、价廉、称量方便等优点。其配方如下:做血清醋纤膜电泳为M/10 KH2PO434ml;M/20 Na2BB4O766ml加蒸馏水25ml,110V电压60一70分钟(春天60分钟、冬天70分钟),在做血清线电泳等时系用工作液,即用上面的磷酸一硼酸缓冲液2份加蒸馏水3份配成。试验结果:血清醋纤膜电泳(见图1),线电泳(见图2),免疫电泳(见图3),对流电泳(见图4),琼脂糖扩散(见图5),效果均
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