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da xi yang zuo xiong yu huo ti he li ti fen mi xing lei gu chun ji su de yan jiu
Pages: 532-538
Year: Issue:  6
Journal: Oceanologia et Limnologia Sinica

Keyword:  类固醇激素大西洋鲑离体培育分泌性大麻哈鱼雄烯二酮培养液促性腺激素性类固醇激素羟孕酮;
Abstract: The levels of serum progesterone, 17α-hydroxyprogesterone, androstenedione, testosterone 11-oxotestosterone (11-OT) and 17α-hydroxy-20β-dihydroprogesterone (17α20βP) in mature male (with milt) Atlantic salmon (Salmo salar) were measured with radioimmunoassay. 11-OT level (23.2±7.12 ng/ml) was much higher than testosterone (4.7±1.42 ng/ml ) in October. 17α20βP was also found in the circulating blood with the level of 7.6±2.45 ng/ml in October. But it was less than 0.1 ng/ml in September.Testes from a mature Atlantic salmon (with milt) were cut into small pieces. One gram such testicular fragments were incubated in 3 ml trout balanced saline solution buffered with HEPES-NaOH containing 0.1 or 1 ng/ml chum salmon (Oncorhynchus keta) gonadotrophin (GTH). Incubation media were taken at intervals during six days incubation and measured. GTH stimulated an obvious release of 11-OT and the level was considerably high. Both testosterone and androstenedione levels rose slowly. Both progesterone and 17α-hydroxyproges-terone levels were extremely low in the medium. 17α20βP level rose quickly after two days incubation. The levels of steroids in the medium did not show GTH dose-dependent. From those results, it shows that 11-OT is a more important and active steroid, which responses to GTH. 17α20βP may be involved in spermiation. Progesterone, 17α-hydroxyprogesterone, androstenedione and testosterone may be the precursors of 17α20βP and 11-OT.
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