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ban zuo su zhong du hou zhan chong de zu zhi bing li bian hua
Pages: 824-834
Year: Issue:  6
Journal: Acta Entomologica Sinica

Keyword:  组织病理变化中毒后斑蝥素死亡期脂肪体粘虫育精囊中毒期血球数增殖;
Abstract: During the course of this study, the sixth instar larvae of Leucania separata (Walker)were used as the matertal to test the toxicity of cantharidin. Some histopathologicalinvestigations have been made on the blood, mid-gut, thoracic ganglia, fat-bodies, Mal-pigian tubes and testis. Normal and poisoned sections were placed side by side on thesame microscopical slide. Sections for general observations were stained with Heiden-hain's iron hematoxylin and eosin, silver nitrate, gold chloride, or gentian violet; theywere also stained with methyl green-pyronin, Schiff's reagent or periodic acid-Schiff re-agent for histochemical studies. The blood cell counts in this experiment were bothmade immediately after the intoxication and before death of the insects, the result show-ing a progressive decrease (from 737 to 135) of cell per μl. It usually appeared to con-tain very few blood cells which came together to form clumps. The symptoms of cantharidin poisoning are mainly due to nervous injuries. Thespecimens treated with cantharidin passed three successive stages: paralytic, swooning anddeath. In these specimens, the epithelial cells of the mid-gut, the Golgi bodies of thethoracic ganglia cells, the testis of the male reproductive system, the Malpigian tubesand fat-bodies are all greatly altered, resulting in fragmentation, pulverization, and dis-integration.
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