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zhong guo dong fang ling zuo bombina orientalis pi fu zhong c-12-b zuo de fen li yu jian ding
Author(s): 
Pages: 202-206
Year: Issue:  2
Journal: Acta Physiologica Sinica

Keyword:  Bombina orientalisC_(-12-h) peptidebombesin;
Abstract: Methanol extracts of the fresh skin of China amphibians (Bombina orientalis)caused contraction of the smooth muscles of rat in vitro.The crude extracts wereisolated by column chromatography of alkaline alumina,and a bioactive fractionwas obtained from the 80% ethanol eluate.Submitting the fraction on SephadexG-15 column,we found that the early eluate C_(-12),showing more bioactivity,was inactivated by hydrolysis with chymotrypsin,but not completely antagonizedby cyproheptadine.C_(-12)was further analyzed by reversed phase-high perfor-mance liquid chromatography (RP-HPLC).The elution time of C_(-12-h) peak wasthe same as that of bombesin,and these two peptides had the same amino acid com-position.These experimental results suggest that C_(-12-h) peptide is most pro-bably bombesin.
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