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Author:
Journal / Book Title:
Year:
Volume:
Issue:
rhgh de shou ti jie he huo xing yan jiu
Author(s):
Ma Xin
,
et al Dept.of Biochemistry
,
Shandong Medical University
Pages:
91
-
93
Year:
1991
Issue:
2
Journal:
Journal of Shandong University(Health Sciences)
Keyword:
Recombinant human growth hormone
;
Receptors binding
;
Microsomes
;
liver
;
Rabbits
;
Abstract:
重组人生长激素(rhGH)与兔肝微粒体GH受体呈特异性结合。结合特性及竞争取代曲线特征与文献中人垂体GH所得结果完全一致。rhGH与鹌鹑肝微粒体间无结合活性。Scatchard分析所得亲和常数Ka为3.5325×10
9
mol
-1
,与文献报道人垂体GH结果相同。这些结果表明,rhGH有与人垂体GH相同的受体结合活性。
Citations
Scatchard G. The attractions of protiens for small molecules and ions . Annals of the New York Academy of Sciences . 1949
Tsushima T and Friesen HG. Radioreceptor assay for growth hormone . The Journal of Clinical Endocrinology . 1973
Mendelsehn LG. Growth hormone receptors . Life Sciences . 1988
Calvo JC,et al. Measurement of specific radioactivities in iabelled hormones by self-displacement analysis . Biochemical Journal . 1983
Zezulak KM and Green H. The generation of insulin-like growth factor-1-sensitive cells by growth hormone action . Science . 1986
Lowry OH,et al. Protien measurement with the folin phenol reagent . Journal of Biological Chemistry . 1951
Kolata G. New growth industry in human growth hormone . Science . 1986
Salacinski PP,et al. Iodination of protiens glycoproticns,and peptides using a solid-phase oxidizing agent,1,3,4,6-tetrachloro-3α,6α-diphenyl glycoluril(Iodogen) . Analytical Biochemistry . 1981
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