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li ti tu er dong mai zhong nei fei zuo de han liang ji qi zuo yong de yan jiu
Pages: 202-209
Year: Issue:  2
Journal: Acta Physiologica Sinica

Keyword:  endogenous opiate-like substancenaloxonepronasebioassayradioimmunoassayrabbit ear artery strip;
Abstract: The existence of opiate receptors in the peripheral artery wall has been wellestablished. However, no evidence of opiate-like substances (OLS) in such tissueshas yet been confirmed. In the present study, measurement of OLS content bybioassay and leu-enkephalin (Leu-enk) by radioimmunoassay in the rabbit earartery strip has been carried out. The bioassay and radioimmunoassay give re-spectively a mean value of 377.20±118. 0 and 40. 51±6.1pg/mg of wet tissue,indicating that a substance with OLS bioactivity and Leu-enk immunoreactivitymust be present in the blood vessel walls. By means of the actions of naloxone (an opiate receptor antagonist) and pronase(a peptidase), the effects of OLS (or Leu-enk) on the activity of artery strips are alsostudied. Afer the opiate receptors were blocked by naloxone, the contractions ofartery strips to electrical field stimulations were distinctly augmented. Moreover,these contractions were also augmented markedly when the artery strips were pre-treated with pronase. It is suggested that the endogenous OLS (or Leu-enk) evokedby electrical field stimulations has an inhibitory effect on the contracting activity ofartery strips. Some preliminary results also indicate that both presynaptic inhibi-tion of release of noradrenaline and postsynaptic inhibition of smooth muscle mayall take part in the effect of OLS (or Leu-enk) on the rabbit ear artery.
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