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NORCANTHARIDIN ENHANCED THG EXPRESSION OF IκBα AND INKIBITED THE PROLIFERATION OF Bel 7402 CELLS in vitro
Author(s): 
Pages: 155-158
Year: Issue:  2
Journal: ACTA ANATOMICA SINICA

Keyword:  去甲斑蝥素(NCTD)NF-κBIκBα核转录因子;
Abstract: Objective In order to study the antitumor mechanism of norcantharidin(NCTD),the effects of NCTD on nuclear transcription factor kappa B(NF-κB) and the inhibitor of NF-κB(IκBα) of the human hepatic carcinoma cells (Bel 7402) were investigated. Methods Exponentially growing Bel 7402 cells were used for plotting the growth curve,detecting the ability of forming cell colony and MTT test.Cel ls were stained by Giemsa,and used for detecting the expression of p65(a subunit of NF-κB) and IκBα by immunocytochemical method and Western blot.Results Addition into the culture,NCTD inhibited the growth of Bel 7402 i n a concentration-and incubation time-dependent manner.Immunocytochemical stai n and Western blot showed that NCTD enhanced the expression of IκBα in cells o bviously. Conclusion NCTD inhibited the proliferation of Bel 7402 cells in a dose-and time-dependent manner.The mechanism may be related to the increasin g expression of IκBα,the inhibitor of NF-κB,sequentially suppress the activi ty of NF-κB,which is necessary for the survival of tumor cells.
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