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ULTRASTRUCTURAL IDENTIFICATION OF A NOVEL MEMBRANE STRUCTURE ON PERITONEAL SURFACE
Author(s): 
Pages: 177-179
Year: Issue:  2
Journal: ACTA ANATOMICA SINICA

Keyword:  腹膜透析腹膜形态学腹膜转运电镜;
Abstract: Objective To testify if there is a stagnant layer on theperitoneal surface of rats and try to find out the components of this layer. Methods Normal rat peritonea were taken from four SD rats. Th e tissues were immediately fixed by using one of the following fixatives for 24 hours before conventional tissue processing:(1)2.5% glutaraldehyde and 2% polyf ormaldehyde(control);(2)2.5% glutaraldehyde and 0.5% cetylpyridinum chloride to fix mainly the glycosaminoglycan(GAG);(3)2% OsO4 to fix the phospholipids (PH1);(4)4% OsO4 (PH2) and (5) 3% tannic acid(Tannic Acid Group). Results In the control, the peritoneal mesothelium was covered with microvilli whereas no surface layer was observed. However, in the GAG grou p, there appeared to be a discontinuous amorphous layer covering the mesothelial cells. In the PH1 group, a continuous amorphous layer covered the peritoneal su rface and its thickness depended on the abundance of microvilli. This layer was much better preserved in the PH2 group with thickness up to 10μm. In the Tannic Acid Group, not only this stagnant layer could be easily seen, we could but als o observe there were a lot of lamellar bodies in the layer and inside the mesoth elial cells. Conclusions Our results demonstrate that for the first time(1) normal peritoneal surface is covered by a pseudo-membrane layer which is easil y dissolved and therefore not seen in conventional tissue processing; (2) This p seudo-membrane layer consists of at least phospholipids and glycosaminoglycan(m ost possibly hyaluronan). We postulate that this layer may be an important barri er to peritoneal transport.
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