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A STUDY OF THE EXPRESSION OF CNTF AND ITS RECEPTOR IN ASTROCYTES
Author(s): 
Pages: 187-189
Year: Issue:  2
Journal: ACTA ANATOMICA SINICA

Keyword:  纤维型星形胶质细胞原浆型星形胶质细胞睫状神经营 养因子睫状神经营养因子受体α;
Abstract: Objective To study the mRNA expression of CNTF and its rece ptorin primary cultured astrocytes. Methods Using in situ hybridization with digoxigenin-labelle d RNA probes and oligo-nucleotide probes. Results CNTF mRNA expression level was higher in the 0-2A progeni tor cells and the process-bearing astrocytes (fibrous astrocytes), but lower in most of the flat-astrocytes (protoplasmic astrocytes). There was only 5% of th e flat-astrocytes highly expressing CNTF.CNTFRα mRNA was also detected in the flat and process-bearing astrocytes. Conclusion Our results indi cate that the expression level of CNTF in two kinds of astrocytes is different, but the expression level of CNTFRα is the same.
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