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Observation on the effects of hemagglutinating activity of ND,EDS-76 viruses under different conditions
Author(s): 
Pages: 94-95
Year: Issue:  2
Journal: JOURNAL OF LUOYANG AGRICULTURAL COLLEGE

Keyword:  NDEDS-76病毒血凝性;
Abstract: 分别观察了不同稀释液、不同感作温度、不同红细胞浓度及不同感作时间对ND、EDS-76病毒HA效价、血凝特性及血凝解脱现象的影响.结果表明,在进行HA试验时,以PH7.2的PBS液为稀释液、0.5%鸡红细胞为指示系统、在37?℃下感作20?min或室温下感作30?min为最适试验条件.各样品在0.5%红细胞下测得的HA效价比0.8%红细胞时平均高0.25~0.5个滴度,其它情况下测得的HA效价基本相同,但红细胞的凝集状态及观察结果的时间有所不同.其中,感作温度越高(37?℃),出现结果越早,且凝集现象越明显,但解脱作用发生得也越快.ND病毒所引起的血凝现象仅可持续2?h左右,而EDS-76病毒引起的血凝现象可持续至24?h不发生解脱,这一特性可作为鉴别2种病毒的1个指标.
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