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Expression and autophosphorylation analysis of XA21 and PI-D2 protein kinases in Saccharomyces cerevisiae
Author(s): 
Pages: 1009-1012
Year: Issue:  6
Journal: ACTA MICROBIOLOGICA SINICA

Keyword:  水稻白叶枯病稻瘟病抗病基因酿酒酵母;
Abstract: 白叶枯病和稻瘟病是最主要的水稻病害.Xa21是水稻白叶枯病抗性基因,Pi-d2是稻瘟病抗性基因,二者都编码类受体激酶蛋白质.在前期研究中,曾系统地研究了细菌中表达XA21激酶蛋白质的生化活性.在此实验中利用真核表达系统酿酒酵母对Xa21和Pi-d2编码的蛋白激酶进行了表达、纯化及自我磷酸化活性分析,为进一步的生化分析、蛋白质-蛋白质相互作用研究、底物筛选等奠定了基础.
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