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Expression of Hypodermin B Gene of Hypoderma lineatum in Escherichia coli
Author(s): 
Pages: 683-688
Year: Issue:  6
Journal: JOURNAL OF INNER MONGOLIA UNIVERSITY(ACTA SCIENTIARUM NATURALIUM UNIVERSITATIS NEIMONGOL)

Keyword:  纹皮蝇Hypodermin B原核表达Western blotting;
Abstract: 从纹皮蝇Ⅰ期幼虫中提取总RNA,RT-PCR扩增纹皮蝇Hypodermin B(HB)基因.将扩增基因进行克隆测序,构建原核表达载体pET30-HB,转化大肠杆菌BL21(DE3).用IPTG诱导表达后,得到表达量达全菌蛋白30%以上的特异性蛋白.SDS-PAGE分析表明表达产物主要以包涵体形式存在.Western blotting检测结果表明,获得的重组蛋白为重组Hypodermin B,具有较好的反应原性.
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