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zhi dao da shui yu shi fei de sheng yang liang sheng wu ce yan fa de yan jiu
Pages: 539-546
Year: Issue:  6
Journal: Oceanologia et Limnologia Sinica

Keyword:  生氧量生物测验法水域限制性营养浮游植物密度大泽山水库藻种施肥养鱼限制性营养盐水样;
Abstract: The oxygen evolution method, a bioassay technique determining the limiting nutrients and the optimum dosages for artificial fertilization, was studied in Dazeshan Reservoir, Ping-du County, Qingdao, during 1985-1987 to obtain an optimun fish farming with artificial fertilization.The general procedure of the method was as follows: water samples collected from 0.5--1.0m depth were filtered through No. 20 bolting cloth, and filled into 500 ml colorless glass bottles; then predetermined concentrations or combinations of nutrients and algae culture were added to each bottle. All treatments and the control were triplicate. These bottles were hung horizontally at the depth half way of the Secchi disk measurements; and the dissolved oxygen (DO) of each bottle was determined after a cerlain period of incubation. The nutrients added in those treatments which have larger DO increments than the controls were considered limiting, and the combination or level of the nutrients added in that treatment which has the highest DO increment was considered to be the optimum.This method is satisfactory in both sensitivity and repeatability and as effective as the batch culture method, hence applicable and reliable for guiding artificial fertilization in large water bodies. This method also proves that at the density of 2-5 million phytoplankton per litre as in this reservoir, the inoculation of algae is not indispensable, and the "in situ" incubation time of the samples should be two days.
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