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fpv282e4 zhu bamhi7 3kb pian duan de ya ke long
Author(s): 
Pages: 64-67
Year: Issue:  2
Journal: Journal of Jilin Agricultural University

Keyword:  亚克隆FPVFPV非必需区;
Abstract: 选用FPV282E4株BamHI7.3kb含非必需区片段,经XbaⅠ酶切,将A、B、C3个片段亚克隆于KS(—)质粒中,同时使D片段自身环化,获得KSFa、KSFb、KSFc、pUCFd4个重组克隆,经酶切鉴定证明亚克隆正确。为以FPV基团组非必需区的BamHI7.3kb片段中BglⅡ片段所在区域构建重组载体质粒和体内重组表达外源基因的研究以及非必需区的核苷酸序列分析奠定基础
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