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fang zhi xue xi chong bing yao wu de yan jiu . wei liang ti de bi se ce ding fa
Pages: 127-132
Year: Issue:  2
Journal: Acta Physiologica Sinica

Keyword:  比色测定法微量锑醋酸硫酸纳干涉毫升生物样品血吸虫病亚硫酸钠提取液;
Abstract: A procedure for colorimetric microdetermination of Sb in biological materials by means of rhodamine B and amyl acetate is established:To the sample add 3 ml of concen- trated H_2SO_4,and digest with concentrated HNO_3.Following the complete destruction of organic matter add 3 ml of 1% Na_2SO_3 and heat until fumes of SO_3 evolve,thus the Sb is reduced to trivalent.On cooling,add 4 ml of 6 N HCl and 2% Ce(SO_4)_2 to slight excess, so the Sb is oxidized to pentavalent.Dilute to 24 ml with H_2O.Extract the Sb with 5 ml of amyl acetate.After complete removal of the aqueous layer,add 9 ml of 2.5 N HCl and 1 ml of 0.2% rhodamine B and shake.If the aqueous layer becomes colorless or only faintly colored, add more rhodamine B.Then centrifuge and take the acetate phase for color measurement, using yellow-green filter.If the color is too intense that does not obey Beer-Lambert law, dilute the extract with amyl acetate previously shaken with 2.25 N HCI. The advantages of this procedure are:(a)high sensitivity;(b)no interference from iron or ceric sulfate;(c)color stable;(d)high extracting power of amyl acetate which is less volatile; (e)easily to be mastered and(f)high duplicability. The influences of amounts,concentrations and order of adding reagents are studied and discussed.
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