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que xue he yao wu yu chu li dui mo ni que xue zai guan zhu pei yang xin ji xi bao dan bai ji mei c huo xing de ying xiang
Author(s): 
Pages: 78-81
Year: Issue:  S1
Journal: Academic Journal of Second Military Medical University

Keyword:  心肌细胞再灌注损伤心肌细胞保护蛋白激酶C;
Abstract: 目的:通过观察短暂缺血及药物预处理对模拟缺血再灌注培养心肌细胞蛋白激酶C(PKC)活性的影响,探讨PKC在预处理中的作用机制。方法:培养心肌细胞分别经短暂缺血及佛波酯、去甲肾上腺素、腺苷预处理,行模拟缺血再灌注,以特异性多肽GS为底物,通过测定掺入GS的γ-32P-ATP放射性活度计算PKC活性。结果:缺血可诱导心肌细胞PKC激活,再灌注抑制PKC活性的继续激活;短暂缺血及药物预处理可引起细胞PKC明显上调,在缺血再灌注过程,PKC活性持续激活,而H7则抑制预处理对PKC的这种持续激活作用。结论:PKC介导的细胞信息传递参与了缺血预处理过程,PKC可能在缺血预处理中起着关键的作用。
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