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Molecular phylogenetic relationship among seven Nemipterus species based on partial S7 ribosomal protein gene sequences
Author(s): 
Pages: 506-513
Year: Issue:  3
Journal: Journal of Fishery Sciences of China

Keyword:  金线鱼属系统发育S7核糖体蛋白基因NemipterusphylogenyS7 ribosomal protein gene;
Abstract: 测定了7种金线鱼属(Nemipterus)及2种锥齿鲷属(Pentapus)鱼类S7核糖体蛋白基因(S7基因)内含子1部分序列,以二线眶棘鲈(Scolopsis bilineatus)做为外类群初步探讨了其分子进化关系.测序所得S7部分序列为734~746 bp,序列比对后得到同源序列743 bp.其中保守位点386个,变异位点351个,简约性信息位点289个.A+T含量(54.1%)高于G+C含量(45.9%).基于Kimura 2-Parameter模型计算出7种金线鱼的遗传距离为0.042~0.294.S7序列存在大量碱基插入与缺失,其中日本金线鱼(Nemipterus japonicus)与苏门答腊金线鱼(N. mesoprion)在第167、182、474、608、662 bp位置,金线鱼(N. virgatus)与印度洋金线鱼(N. bipunctatus)第227、332、401、604 bp的位置具有相同的碱基插入缺失特征,且具有种类特异性.最后利用最大似然法(ML)与贝叶斯分析(BI)构建分子系统进化树,7种金线鱼聚在一起,其中日本金线鱼与苏门答腊金线鱼聚为一支,金线鱼、深水金线鱼和印度洋金线鱼聚成一支.结论认为需要结合多方面的形态与分子证据,才能进一步明确金线鱼属鱼类的系统进化关系.%Nemipterus species, belonging to the family Nemipteridae, order Perciformes, are widely distributed in the tropical and subtropical waters of the Indo-Western Pacific. Morphologically, a number of closely related Ne-mipterus species share similar external coloration and patterns, resulting in difficulty identifying and classifying them based on morphological evidence alone. Despite this, priorevaluation of Nemipterushas primarily relied on morphological data, with few molecular phylogenetic data currently available for this genus. We analyzed the mo-lecular phylogenetic relationship among seven common Nemipterus species collected from the Indo-Western Pa-cific. Partial sequences of the 1st intron of the S7 ribosomal protein gene were obtained for seven Nemipterus and two Pentapus species. We used sequence data from Scolopsis bilineatus as an outgroup. Partial S7 sequences ranging from 734 bp to 746 bp and homologous sequences of 743 bp were obtained for phylogenetic analysis after alignment. Of the 743 bp aligned sequences, 386 sites were conservative, 351 sites were variable, and 289 were parsimony-informative. The content of A+T (54.1%)was lower than that of G+C (45.9%). The genetic distance among the seven Nemipterus species, calculated using a Kimura two-Parameter model, were between 0.042 and 0.294. We observed a number of insertions and deletions in the S7 ribosomal protein gene sequences. The inser-tions and deletions at 167 bp, 182 bp, 474 bp, 608 bp, 662 bp within N. japonicus and N. mesoprion, and at 227 bp, 332 bp, 401 bp, and 604 bp within N. virgatus and N. bipunctatus were identical. We constructed molecular phy-logenetic trees using the maximum likelihood and Bayesian inference methods. The seven Nemipterus species were clustered together as a monophyletic group. N. japonicus and N. mesoprion were clustered in one clade, and N. virgatus, N. bipunctatus and N. bathybius were clustered in a separate clade. These phylogenetic relationships were consistent with their morphological classifications. However, the phylogenetic position of N. bipunctatus was inconsistent with Mohds’ hypothesis under which Nemipterus species were divided into two morphologically dis-tinct groups according to the length of upper and lower caudal fin lobes. The two lobes are of equal length in N. bipunctatus, whereas N. virgatus and N. bathybius have an elongated trailing filament from their upper caudal fin and the upper fin lobe is longer than the lower. Our results suggest acombination of more morphological and mo-lecular evidence is needed for further phylogenetic studies of the Nemipterus genus.
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