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Study on the HPLC specific chromatogram of flavonoids in dendranthema indicum
Author(s): 
Pages: 182-185
Year: Issue:  3
Journal: Chinese Journal of Hospital Pharmacy

Keyword:  Dendranthema indicum HPLC specific chromatogram;
Abstract: 目的:建立神农香菊药材黄酮类成分HPLC特征图谱。方法:采用高效液相色谱法,色谱柱为Dionex-Acclaim 120 C18(250mm×4.6mm,5μm);流动相为磷酸盐缓冲溶液(pH2.5)-甲醇(梯度洗脱);流速0.5mL·min-1;柱温35℃;检测波长350nm。结果:建立了神农香菊药材黄酮类成分HPLC特征图谱,标定了17个共有特征峰,方法学考察符合特征图谱技术要求。结论:本方法稳定,可靠,精密度高,重复性好,可为神农香菊药材质量标准的制定提供科学依据。
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