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Cloning of cDNA for flounder Interferon Regulatory Factor (fIRF) and its primary expression in prokaryocyte
Author(s): 
Pages: 10-13
Year: Issue:  2
Journal: JOURNAL OF FISHERY SCIENCES OF CHINA

Keyword:  牙鲆干扰素调节因子分子克隆原核表达;
Abstract: 采用逆转录—聚合酶链式反应(RT-PCR)方法,从牙鲆(Paralichthys olivaceus)肝脏总RNA中扩增出干扰素调节因子(fIRF)的核心区序列。定向插入质粒pUC18,克隆的牙鲆IRF cDNA序列分析表明,与Yabu报道的牙鲆IRF cDNA相比有1个碱基差异,但与推断的氨基酸序列完全一致。将牙鲆IRF的核心区序列cDNA定向插入原核表达载体pBV220,构建成牙鲆IRF表达载体pBVfIRF22。SDS-PAGE分析表明,经42℃诱导,含pBVfIRF22质粒的大肠杆菌可表达一分子量约12 000 的特异蛋白。
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