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Autologous dendritic cells eliciting cytotoxicity of bone marrow cells againstc hronic granulocytic leukemia
Pages: 64-67
Year: Issue:  2

Keyword:  树突细胞细胞毒性免疫白血病髓样慢性;
Abstract: Objective To investigate the activity of bone marrow cells activated by autologous dendrit ic cells(DC) to mediate cytotoxicity against chronic granulocytic leukemia(CGL) cells. Methods DC were separated from bone marrow mononuclear cells(BMMNC) of two CGL patients in hematological remission and harvested after 3 d ays of culture in IMDM containing autologous plasma, rhGM-CSF and rhTNFα at 37 ℃, 5% CO2 humidified atmosphere. BMMNC obtained from the patients were divi ded into 3 groups to set up Dexter systems: the control group, rhIL-2 contain ing, and the third group having DC added at day 4. After 10 days of culture, non -adherent cells were harvested and the changes of immunological phenotype and t he percentage of P210 positive cells were analyzed. The cytotoxicity were assaye d with two-colour flow cytometry. The non-adherent cells from all the 3 system s served as effector cells, those from control system as target cells. Res u lts The cytotoxic activity against target cells was greater in the DC- activated effector cells than that in rhIL-2-activated ones. The percentages of death cells in target cells were 63.12% versus 42.59%(case 1) and 61.60% ver sus 21.46% (case 2), respectively. In addition, there was a marked increase in the death cell percentage in the DC-activated effector cells themselves after i ncubation with target cells. This phenomenon was not found in the rhIL-2-activated effector cells. The percentage of P210 positive cells was significantly lower in non-adherent cells after 10 days of culture in Dexter s ystem, comparing with that in non-cultured BMMNC. The least P210 positive cells were found in those cultured with DC and the less in those with rhIL-2. Conclusion Autologous DC were able to activate bone marrow cells to generate cytotoxicity against CGL cells. Their effect was greater than that of rhIL-2. These activated bone marrow cells might mediate graft versus leukemia e ffect in vivo.
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