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Breeding Methods of the New Strain of Genetically Modified Hetao Melon and its Ecological Suitability
Author(s): 
Pages: 119-121
Year: Issue:  S1
Journal: Acta Agriculturae Boreali-Sinica

Keyword:  ACS基因 PCR扩增技术 花粉管通道法 CTAB法 生态适应性;
Abstract: 1-氨基环丙烷-1-羧酸合成酶(ACS)是高等植物中乙烯合成的关键酶。以成熟河套蜜瓜果实的RNA为模板,经反转录和PCR扩增合成和克隆了河套蜜瓜ACS基因cDNA片断(627 bp),将ACS基因cDNA反向构建到植物表达载体pGA643,配制成DNA溶液后用花粉管通道法导入外源基因,对转基因河套蜜瓜的叶片用CTAB法提取总DNA,PCR扩增检测,结果表明某些果实的部分种子已整合外源基因。目前,该转基因新品系正在进行生态适应性研究及“环境释放”和“生产性试验”的安全性评价。
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