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Establishment of Tissue Culture System of Pinellia ternata(Thunb.) Breit.
Author(s): TAO Mi-lin, LIU Qing-bo, HUANG Hong-mei
Pages: 113-
117
Year: 2013
Issue:
4
Journal: Northern Horticulture
Keyword: 半夏; 组织培养; 顶芽; 块茎;
Abstract: 以半夏块茎和顶芽为试材,对顶芽愈伤组织诱导、顶芽愈伤组织不定芽分化、块茎不定芽分化和不定芽诱导生根的最佳条件进行了研究。结果表明:半夏最佳顶芽愈伤组织诱导培养基为MS+6-BA 2.0mg/L+NAA 0.2mg/L;最佳顶芽愈伤组织不定芽分化培养基为MS+6-BA 1.0mg/L+NAA 0.2mg/L;最佳半夏块茎不定芽分化培养基为MS+6-BA 1.0mg/L+NAA 0.2mg/L;最佳不定芽诱导生根培养基为MS+2,4-D 2.0mg/L+KT 1.0mg/L。
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