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The Changes of T Lymphocytes Following Focal Cerebral Ischemia in Rat Examined by Flow Cytometry
Author(s): LIN Zhen-zhen, SHAO Bei, JIN Kun-lin, WANG Liu-qing, JIANG Hui-gang, XIAO Li, ZHANG Hongxia
Pages: 146-
150
Year: 2013
Issue:
2
Journal: Chinese Journal of Clinical Neurosciences
Keyword: 缺血性脑卒中; 淋巴细胞; 流式细胞技术; 大鼠;
Abstract: 目的 使用流式细胞技术分析缺血性脑卒中后大鼠脑组织及血标本内T细胞的变化.方法 应用电凝阻断大脑中动脉,建立局部脑缺血模型.大鼠随机分为缺血性脑卒中组和假手术组,分别应用流式细胞技术分析术后72 h脑组织及血标本内CD3+细胞的变化.结果 大鼠脑缺血后72 h,脑组织内CD3+细胞比例升高,血标本内CD3+细胞比例未见明显升高.结论 流式细胞技术成功检测脑组织内淋巴细胞变化.T细胞可能在大鼠脑缺血损伤中发挥重要作用.
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