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Effects of Soil Factors on Population Dynamics of Clonostachys rosea 67-1 in Soil
Author(s): 
Pages: 97-103
Year: Issue:  1
Journal: Chinese Journal of Biological Control

Keyword:  粉红粘帚霉 实时荧光定量PCR 土壤因子 消长动态;
Abstract: 应用实时荧光定量PCR技术,通过室内营养钵土壤人工接种粉红粘帚霉67-1菌株,研究土壤温度、pH、含水率对生防菌粉红粘帚霉在土壤中消长动态的影响。结果表明:在不同温度、含水率和酸碱度的土壤中,菌株67-1种群动态变化曲线基本一致,接种5 d前土壤中67-1拷贝数明显减少,10 d后拷贝数逐渐增加,20 d时达到最高,并维持在这一水平。土壤因子对菌株67-1的定殖量具有显著影响,接种相同时间30℃时土壤中菌株67-1拷贝数增加最快,其次是20℃,10℃最低;土壤pH值为5.0时拷贝数最高,pH为8.0时拷贝数较低,pH为4.0时拷贝数最低;土壤含水率为15%时菌株67-1拷贝数高于含水率25%,但二者差异不显著,表明菌株67-1在土壤中的扩增受土壤含水率影响较小。
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