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The effect of autophagy on Streptococcus pneumoniae infections of lung epithelial cells and its mechanism
Author(s): 
Pages: 2496-2498
Year: Issue:  15
Journal: The Journal of Practical Medicine

Keyword:  肺炎球菌感染LC3肺泡Ⅱ型上皮细胞3MA自噬体;
Abstract: 目的:检测微管相关蛋白轻链-3(LC3)在肺泡Ⅱ型上皮细胞A549上的表达情况,及3-甲基腺嘌呤(3MA)刺激后对其表达的影响,为研究自噬体在抵抗肺炎链球菌感染过程中的保护作用奠定基础.方法:体外培养肺泡Ⅱ型上皮细胞A549,在肺炎链球菌感染A549细胞12h时用倒置显微镜拍照并提取RNA,采用RT-PCR的方法检测LC3 mRNA的表达情况.同时检测对照组、3MA组、Spn组和3MA+ Spn组上清液乳酸脱氢酶(LDH)的OD值.结果:RT-PCR检测LC3 mRNA有明显表达,3MA可以抑制LC3的表达.LDH检测显示Spn组和3MA+ Spn组上清液LDH的OD值数据两两比较差异有统计学意义(P<0.05).结论:LC3在肺泡Ⅱ型上皮细胞中显著表达,3MA可以抑制细胞的自噬作用.加3MA后,肺炎链球菌感染肺泡Ⅱ型上皮细胞坏死增加,提示自噬体在抵抗肺炎链球菌的感染过程中起一定的保护作用.
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