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Vertical transmission of baculovirus and expression of baculovirus-mediated green fluorescent protein gene in successive generations of Helicoverpa armigera
Author(s): 
Pages: 1-8
Year: Issue:  1
Journal: ACTA ENTOMOLOGICA SINICA

Keyword:  绿色荧光蛋白基因重组棉铃虫核型多角体病毒垂直传播;
Abstract: 用转座/穿梭系统构建了携带绿色荧光蛋白基因(gfp)的重组棉铃虫核型多角体病毒rHa-FGP,以其多角体添食感染棉铃虫3龄幼虫,室内饲养3代,各代均可见自然光下发绿色荧光的棉铃虫幼虫,其中子代不再重复感染.F0、F1F2代发绿色荧光的棉铃虫幼虫所占百分比分别为34%、20%、8%.提取虫体内的病毒多角体DNA,以PCR和斑点杂交鉴定表明,gfp不仅在亲代棉铃虫体内正常表达,而且在子代幼虫中表达,HaNPV通过卵实现了垂直传播.
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