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Random amplified polymorphic DNA of resistance to dimehypo andcartap in diamond-back moth, Plutella xylostella
Author(s): 
Pages: 15-20
Year: Issue:  1
Journal: ACTA ENTOMOLOGICA SINICA

Keyword:  小菜蛾杀虫双杀螟丹随机扩增多态性DNA;
Abstract: 利用室内选育119代的抗杀虫双和抗杀螟丹小菜蛾Plutella xylostella品系和敏感品系,通过反复回交建立近等基因系.用Operon公司合成的80个引物对抗杀虫双近等基因系和抗杀螟丹近等基因系小菜蛾基因组DNA进行PCR扩增;在抗杀虫双近等基因系中有3个引物分别产生了1条特异扩增带,2个引物分别产生了2条特异扩增带,1个引物产生3条特异扩增带;在抗杀螟丹近等基因系中,有4个引物分别产生了1条特异扩增带,1个引物产生了2条特异扩增带.在培育近等基因系的多次回交过程中,与抗性有关的遗传因子被逐步置换到敏感品系的基因组中,因此可以认为这些特异带与小菜蛾对杀虫双和杀螟丹的敏感性有关.
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