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Development and characterization of monoclonal antibodies to the white-backed planthopper Sogatella furcifera
Author(s): 
Pages: 21-26
Year: Issue:  1
Journal: ACTA ENTOMOLOGICA SINICA

Keyword:  白背飞虱单克隆抗体杂交瘤技术;
Abstract: 应用杂交瘤技术,制备出4株高度特异性的白背飞虱单克隆抗体,分别命名为WPH-1H9、WPH-2B6、WPH-2E12和WPH-3F12.这些抗体与其它8种昆虫未发生交叉反应,其中WPH-2B6可与白背飞虱所有虫态发生反应,其余3株只与卵和雌成虫发生反应.应用免疫双扩散法鉴定抗体类型及亚类,结果表明:WPH-2B6为IgG2b亚类,其余均为IgGl亚类.SDS-聚丙烯酰胺凝胶电泳和Western blot印迹分析表明,白背飞虱抗原主要由分子量分别为182、116、66.2及40 kD的4个多肽组成,其中WPH-2B6与182、116 kD的多肽结合,其余3株的单抗只与116 kD的多肽具有亲和性.最后对这些单克隆抗体在捕食作用研究中的应用潜力进行了讨论.
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