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Correlation between 20-hydroxyecdysone levels and cuticulogenesis in nymphs of Haenaphysalis longicornis
Author(s): 
Pages: 46-50
Year: Issue:  1
Journal: ACTA ENTOMOLOGICA SINICA

Keyword:  长角血蜱若虫20-羟基蜕皮酮表皮发生;
Abstract: 用高效液相色谱和放射免疫分析对长角血蜱Haemaphysalis longicornis若虫发育期整体蜕皮激素(20-E)含量变化进行了测定,并用扫描电镜对其表皮结构及其发生进行了观察.20-E存在于若虫整个发育期,饥饿、吸血和饱血后前4天,激素水平低(0.71~1.30 ng/头);饱血后6天明显增加,此时皮层溶离开始,蜕皮间隙形成;饱血后7天继续增加;饱血后8天,20-E含量达到高峰(8.70 ng/头),此时若虫开始沉积新的上表皮;高峰后,20-E含量急剧下降,直到蜕皮前保持低水平,此期内沉积新的原表皮,旧表皮被部分吸收.上述结果显示出20-E含量急剧增加及其高峰分别与启动皮层溶离和新的上表皮沉积相吻合,20-E与新的原表皮沉积无关.
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