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Target of hydrochloric acid in acid-treatment of diapausing eggs of Bombyx mori
Author(s): 
Pages: 51-55
Year: Issue:  1
Journal: ACTA ENTOMOLOGICA SINICA

Keyword:  家蚕滞育盐酸处理酯酶A4靶物质;
Abstract: 酯酶A4(EA4)是家蚕卵的滞育生物钟蛋白质.从家蚕C108品种产下后48 h的滞育性卵和盐酸活化处理卵分离纯化出EA4酶蛋白,使用合成的EA4活性多肽抑制因子PIN(氨基酸结构:SIFMTKQHSQ DDIIQHPLDY VEQQIHQQKQ KLQKQTLN),研究了PIN对EA4酶蛋白的作用机制.滞育性卵的EA4酶蛋白和PIN在25℃混合24 h后,用矩阵辅助激光解吸离子质谱法,检测到了二者的结合体,该结合体在盐酸处理后消失;盐酸活化处理蚕卵的EA4酶蛋白和合成PIN之间没有出现这种结合体.体外25℃,滞育性蚕卵EA4的ATPase特征性活性峰在6.5 h后出现,而盐酸活化处理蚕卵的EA4在1.5 h后出现活性峰值.盐酸处理可能通过解除PIN对EA4的抑制作用,在短时间内激活EA4酶蛋白,从而活化滞育性蚕卵.
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