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Leptin induces keratinocytes proliferation by JAK2/STAT3 signal transduction pathway
Author(s): 
Pages: 2997-3000
Year: Issue:  18
Journal: The Journal of Practical Medicine

Keyword:  瘦素角质形成细胞JAK2-STAT3信号通路创面愈合;
Abstract: 目的:探讨瘦素对角质形成细胞增殖的作用及其创面愈合中的可能作用及机制.方法:原代培养人表皮角质形成细胞并鉴定.分别观察瘦素对角质形成细胞的时间梯度作用、浓度梯度作用、JAK抑制剂AG490对瘦素促增殖作用的影响,培养24 h后收集细胞行MTT检测、CK17及CK19免疫细胞化学染色.结果:24、48、72 h后,瘦素作用组均较对照组有明显的细胞增殖.在0~400 ng/mL浓度范围,瘦素干预组细胞均较对照组MTT检测OD值升高,有明显的细胞增殖;但在800 ng/mL浓度,瘦素作用组较对照组细胞数更少.CK17及CK19免疫细胞化学染色,瘦素组较对照组着色更深、着色细胞数更多;而AG490+瘦素组较瘦素组着色更浅、着色细胞数更少.结论:100 ng/mL瘦素对角质形成细胞有时间依赖性促增殖作用.0~400 ng/mL浓度范围内,瘦素对角质形成细胞有浓度依赖性促增殖作用.AG490能有效抑制瘦素对角质形成细胞的促增殖作用,提示JAK2/STAT3信号途径参与瘦素的跨膜信号传导.
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