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Determination of Chlorogenic Acid in Boltonia Indica(L.) by HPLC
Author(s): Liang Nieyan, Chen Xuesong
Pages: 21-
22
Year: 2012
Issue:
23
Journal: China Pharmaceuticals
Keyword: 路边菊; 高效液相色谱法; 绿原酸; 含量测定;
Abstract: 目的 建立测定路边菊中绿原酸含量的高效液相色谱法.方法 色谱柱为Agilent SB-C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸,检测波长为348 nm,柱温为35℃.结果 绿原酸进样量在0.026 05 ~1.667 2 μg之间和峰面积具有良好线性关系,线性回归方程为Y=1 846 039X-10 829,r=0.999 8(n=5);测得10批路边菊中的绿原酸平均含量为0.19%.结论 该方法简便可行,重现性好.可作为路边菊的质量控制方法.
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