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SCAR marker for rapid identification of Encarsia formosa Gahan (Hymenoptera: Aphelinidae)
Author(s): 
Pages: 1386-1393
Year: Issue:  12
Journal: Acta Entomologica Sinica

Keyword:  丽蚜小蜂 烟粉虱 快速识别 分子鉴别 SCAR标记 检测阈值;
Abstract: 丽蚜小蜂Encarsia formosa Gahan作为温室粉虱Trialeurodes vaporariorum Westwood和烟粉虱Bemisia tabaci(Gennadius)等粉虱类害虫的优势寄生蜂而备受关注。针对丽蚜小蜂体型微小,难以与其他同域近缘种寄生蜂快速、准确区别的问题,本研究采用SCAR(sequence characterized amplified region,特异性扩增区域)标记技术,筛选出一对丽蚜小蜂特征片段扩增引物(EFZZF/EFZZR),其扩增片段的大小为287bp。种特异性检验结果表明,该对引物只对丽蚜小蜂的基因组DNA具有扩增能力,对其近缘种属寄生蜂如浅黄恩蚜小蜂Encarsia sophia(Girault & Dodd)、海氏桨角蚜小蜂Eretmocerus hayati Zolnerowich & Rose、本地未知种桨角蚜小蜂Eretmocerus sp.、蒙氏桨角蚜小蜂Eretmocerus mundus Mercet、刺粉虱黑蜂Amitus hesperidum Silvertri不具有扩增效果,对丽蚜小蜂的寄主包括不同生物型(B型、Q型、ZHJ-1型和ZHJ-2型)的烟粉虱、温室粉虱以及我国最常见的黑刺粉虱Aleurocanthus spiniferus(Quaintanca)等亦不具有扩增能力。同时,该检测技术灵敏度高,对成虫的最低检出阈值为7.812ng/μL(相当于1/1600头成虫)。研究结果对丽蚜小蜂的种类识别、寄主谱的确定及其有效利用具有重要意义。
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