DETECTION OF HUMAN MALARIA PARASITES BASED ON AMPLIFYING SPECIFIC SSU rRNA GENE FRAGMENTBY NESTED PCR IN SICHUAN, CHINA
Author(s): JIANG Su-hua, LIU Pei-na, ZHU Sheng-hua, LIU Qing, CHEN Jian-ping
Pages: 4-7
Year: 2001 Issue: 1
Journal: JOURNAL OF PRACTICAL PARASITIC DISEASES
Keyword: 间日疟原虫; 恶性疟原虫; 三日疟原虫; 卵形疟原虫; 套式聚合酶链反应; 小亚单位核糖体核糖核酸基因;
Abstract: Aim To detect and identify human malaria parasites by nested PCR. Methods A pair of genus primer and four pairs of species-specific primers are chosen to establish nested PCR system and the targets specific sequence in the SSU rRNA gene are used for detecting blood samples obtained from malaria patients in Sichuan. Results 104bp，102bp and 115bp DNA expected fragments were amplified from the blood samples of 61 patients with P.vivax ,P.falciparum and P.malariae infections respectively. The positive concordance rate between nested PCR and microscopic examination was 100% in detecting P.vivax. In addition , 3 cases of mixed Plasmodium infections were identified by nested PCR. Conclusion The system is specific, sensitive and stable in detecting mixed malatia infections. It would provide a practical tool in diagnosis and epidemiological survey of malaria.
Pages: 4-7
Year: 2001 Issue: 1
Journal: JOURNAL OF PRACTICAL PARASITIC DISEASES
Keyword: 间日疟原虫; 恶性疟原虫; 三日疟原虫; 卵形疟原虫; 套式聚合酶链反应; 小亚单位核糖体核糖核酸基因;
Abstract: Aim To detect and identify human malaria parasites by nested PCR. Methods A pair of genus primer and four pairs of species-specific primers are chosen to establish nested PCR system and the targets specific sequence in the SSU rRNA gene are used for detecting blood samples obtained from malaria patients in Sichuan. Results 104bp，102bp and 115bp DNA expected fragments were amplified from the blood samples of 61 patients with P.vivax ,P.falciparum and P.malariae infections respectively. The positive concordance rate between nested PCR and microscopic examination was 100% in detecting P.vivax. In addition , 3 cases of mixed Plasmodium infections were identified by nested PCR. Conclusion The system is specific, sensitive and stable in detecting mixed malatia infections. It would provide a practical tool in diagnosis and epidemiological survey of malaria.
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