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Molecular Cloning and Sequence Analysis of CbPG Gene from Capsella bursapastoris(L.) Medic.
Author(s): 
Pages: 12-17
Year: Issue:  5
Journal: Acta Agriculturae Boreali-Sinica

Keyword:  CbPG克隆序列特征生物信息学分析;
Abstract: 根据白菜花粉特异的多聚半乳糖醛酸酶(PC)基因BcMF2(EU181170)的全长序列设计特异引物,通过PCR扩增的方法从荠菜中克隆了一个新的BcMF2的同源基因CbPG.该基因与BcMF2在核酸水平上的相似性高达99%.CbPG基因DNA全长为1563 bp,由4个外显子和3个内含子构成,外显子总长为1263 bp,编码420个氨基酸.序列比对结果表明,该基因具有所有PG基因特有的4个保守结构域,并且与花粉中特异表达的PG基因聚为一类,表明该基因可能是与花粉发育相关的PG基因,在花粉萌发和花粉管伸长中起作用.
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